Sample complexity and large dynamic range of protein and peptide concentrations are major hurdles in the analysis of low abundance proteins. Today, for almost any proteomic analysis, robust and reproducible fractionation techniques are key to ensure that MS/MS can identify the maximum number of proteins. At the same time, to avoid inadvertentloss or alteration of critical proteins, it is important that fractionation steps minimize the number of sample manipulations.

OFFGEL electrophoresis separates proteins or peptides according to their isoelectric points, whereby the separated components are recovered in liquid phase. Up- or downstream sample processing steps such as immunodepletion, protein digestion and liquid chromatography can be easily interfaced with this technique for multi-dimensional separations of complex samples.

Description

• Data sheet

Owned and operated by

P/F Fiskaaling

4-gel vertical electrophoresis system.

Description

• Data sheet

Owned and operated by

University of the Faroe Islands